polyclonal rabbit anti human fxr antibody  (Novus Biologicals)

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Effect of L-FABP gene ablation on liver levels of key proteins involved in uptake, synthesis and storage of bile acid precursor cholesterol Liver homogenates of male mice were separated by SDS/PAGE and electroblotted on to nitrocellulose membranes as described in the Experimental section. The membranes were incubated with polyclonal antisera directed against proteins involved in cholesterol uptake (LDL receptor), efflux (SRB-1), synthesis (HMG-CoA reductase) and storage as cholesteryl esters (ACBP, ACAT-2 and ACAT-1). The amount of protein is expressed in arbitrary units (a.u.) with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P <0.05; **, P <0.01. For cholesterol diet versus control diet: #, P <0.05; ###, P <0.001.

Article Snippet: Goat polyclonal anti-human CYP7A1, goat polyclonal anti-human CYP27A1 (sterol 27α-hydroxylase), rabbit polyclonal anti-human FXR (farnesoid X receptor), goat polyclonal anti-human LXRα (liver X receptor α) and goat polyclonal anti-mouse SHP (short heterodimer partner protein) were from Santa Cruz Biotechnology.

Techniques: Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against CYP7A1 (A), CYP27A1 (B) or SCP-x (C) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: #, P<0.05; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-human CYP7A1, goat polyclonal anti-human CYP27A1 (sterol 27α-hydroxylase), rabbit polyclonal anti-human FXR (farnesoid X receptor), goat polyclonal anti-human LXRα (liver X receptor α) and goat polyclonal anti-mouse SHP (short heterodimer partner protein) were from Santa Cruz Biotechnology.

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against cytosolic cholesterol/bile acid transport proteins such as L-FABP (A), SCP-2 (B), caveolin-1 (C), GST (D), or 3α-HSD (E) as described in the Experimental section. The amount of protein was expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P<0.05; **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: ##, P<0.01; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-human CYP7A1, goat polyclonal anti-human CYP27A1 (sterol 27α-hydroxylase), rabbit polyclonal anti-human FXR (farnesoid X receptor), goat polyclonal anti-human LXRα (liver X receptor α) and goat polyclonal anti-mouse SHP (short heterodimer partner protein) were from Santa Cruz Biotechnology.

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against canalicular bile salt translocase proteins such as BSEP (A) and MRP2 (B), or against basolateral/serosal bile salt translocase protein such as OATP-1 (C) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P<0.05; **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet, ###, P<0.001.

Article Snippet: Goat polyclonal anti-human CYP7A1, goat polyclonal anti-human CYP27A1 (sterol 27α-hydroxylase), rabbit polyclonal anti-human FXR (farnesoid X receptor), goat polyclonal anti-human LXRα (liver X receptor α) and goat polyclonal anti-mouse SHP (short heterodimer partner protein) were from Santa Cruz Biotechnology.

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against nuclear regulatory proteins SREBP-1 (A), PPARα (B), FXR (C), LXRα (D) or SHP (E) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): **, P<0.01, ***P<0.001. For cholesterol diet versus control diet: #, P<0.05; ##, P<0.01; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-human CYP7A1, goat polyclonal anti-human CYP27A1 (sterol 27α-hydroxylase), rabbit polyclonal anti-human FXR (farnesoid X receptor), goat polyclonal anti-human LXRα (liver X receptor α) and goat polyclonal anti-mouse SHP (short heterodimer partner protein) were from Santa Cruz Biotechnology.

Techniques: SDS Page, Incubation

Journal: Journal of Biological Chemistry

Article Title: The Farnesoid X Receptor Controls Gene Expression in a Ligand- and Promoter-selective Fashion

doi: 10.1074/jbc.m306422200

Figure Lengend Snippet: FIG. 6. Induction of FXR protein expression by various FXR agonists. A, HepG2 cells were treated with 50 M CDCA at various time points, and the FXR protein was determined by Western blot analysis followed by quantitation with densitometry. Results are nor- malized as fold of control (treated cells versus vehicle), and data are the mean of two determinations. B and C, HepG2 cells were treated with indicated concentration (M) of bile acids (B) or GW4064 (C) for 24 h in DMEM containing 0.5% CS-FBS. At the end of the incubation, nuclear extracts were prepared using a nuclear and cytoplasmic extraction kit according to the manufacturer’s instructions. 20 g of total nuclear pro- teins was separated by 4–20% SDS-PAGE. Western blotting was carried out following the manufacturer’s instructions using the polyclonal rabbit anti-human FXR antibody and donkey anti-rabbit IgG conjugated to horseradish peroxidase and the ECL chemiluminescence kit. DMSO, Me2SO.

Article Snippet: Western blotting was carried out following the manufacturer’s instructions (Amersham Biosciences) using polyclonal rabbit anti-human FXR antibody (catalog number H-130, Santa Cruz Biotechnology).

Techniques: Expressing, Western Blot, Quantitation Assay, Control, Concentration Assay, Incubation, Extraction, SDS Page